The American Anti-Vivisection Society (AAVS) petitioned the National Institutes of Health (NIH) on April 23, 1997, to prohibit the use of animals in the production of mAb. On September 18, 1997, NIH declined to prohibit the use of mice in mAb production, stating that "the ascites method of mAb production is scientifically appropriate for some research projects and cannot be replaced." On March 26, 1998, AAVS submitted a second petition, stating that "NIH failed to provide valid scientific reasons for not supporting a proposed ban." The office of the NIH director asked the National Research Council to conduct a study of methods of producing mAb. In response to that request, the Research Council appointed the Committee on Methods of Producing Monoclonal Antibodies, to act on behalf of the Institute for Laboratory Animal Research of the Commission on Life Sciences, to conduct the study. The 11 expert members of the committee had extensive experience in biomedical research, laboratory animal medicine, animal welfare, pain research, and patient advocacy (Appendix B). The committee was asked to determine whether there was a scientific necessity for the mouse ascites method; if so, whether the method caused pain or distress; and, if so, what could be done to minimize the pain or distress. The committee was also asked to comment on available in vitro methods; to suggest what acceptable scientific rationale, if any, there was for using the mouse ascites method; and to identify regulatory requirements for the continued use of the mouse ascites method. The committee held an open data-gathering meeting during which its members summarized data bearing on those questions. A 1-day workshop (Appendix A) was attended by 34 participants, 14 of whom made formal presentations. A second meeting was held to finalize the report. The present report was written on the basis of information in the literature and information presented at the meeting and the workshop.

This book represents the distillation and critical evaluation of many hundreds of publications relating to the production and use of antibodies. Therefore it is restricted to the "core" techniques of production and handling of antibodies, and their use in studies of antigen analysis, purification and localization.

"Whew! They going to send around here and tie you up and drag you off to Milledgeville. Them fat blue police chasing tomcats around alleys." —Berenice in The Member of the Wedding by Carson McCullers A scathing and original look at the racist origins of the field of modern psychiatry, told through the story of what was once the largest mental institution in the world, by the prize-winning author of Memoir of a Race Traitor After a decade of research, Mab Segrest, whose Memoir of a Race Traitor forever changed the way we think about race in America, turns sanity itself inside-out in a stunning book that will become an instant classic. In December 1841, the Georgia State Lunatic, Idiot, and Epileptic Asylum was founded on land taken from the Cherokee nation in the then-State capitol of Milledgeville. A hundred years later, it had become the largest insane asylum in the world with over ten thousand patients. To this day, it is the site of the largest graveyard of disabled and mentally ill people in the world. In April, 1949, Ebony magazine reported that for black patients, "the situation approaches Nazi concentration camp standards . . . unbelievable this side of Dante's Inferno." Georgia's state hospital was at the center of psychiatric practice and the forefront of psychiatric thought throughout the nineteenth and twentieth centuries in America—centuries during which the South invented, fought to defend, and then worked to replace the most developed slave culture since the Roman Empire. A landmark history of a single insane asylum at Milledgeville, Georgia, A Peculiar Inheritance reveals how modern-day American psychiatry was forged in the traumas of slavery, the Civil War, and Reconstruction, when African Americans carrying "no histories" entered from Freedmen's Bureau Hospitals and home counties wracked with Klan terror. This history set the stage for the eugenics and degeneracy theories of the twentieth century, which in turn became the basis for much of Nazi thinking in Europe. Segrest's masterwork will forever change the way we think about our own minds.

Drug-Induced Liver Injury, Volume 85, the newest volume in the Advances in Pharmacology series, presents a variety of chapters from the best authors in the field. Chapters in this new release include Cell death mechanisms in DILI, Mitochondria in DILI, Primary hepatocytes and their cultures for the testing of drug-induced liver injury, MetaHeps an alternate approach to identify IDILI, Autophagy and DILI, Biomarkers and DILI, Regeneration and DILI, Drug-induced liver injury in obesity and nonalcoholic fatty liver disease, Mechanisms of Idiosyncratic Drug-Induced Liver Injury, the Evaluation and Treatment of Acetaminophen Toxicity, and much more. - Includes the authority and expertise of leading contributors in pharmacology - Presents the latest release in the Advances in Pharmacology series

In accordance with AR 300-15, this manual serves as standard specifications for establishing map identifications and for designing other marginal data on maps prepared by the Army Map Service (AMS) and all other mapping units under the direct control of the Chief of Engineers. This manual also explains the identification on maps of other origins which are to be reprinted or stocked by the above mapping units, and further describes required changes in identifications and other marginal data when reprinting such maps. An appendix describes the identifications and general characteristics of AMS publications dealing with mapping. This manual supersedes all previous instructions on the subject.

By understanding the movement patterns of people, mathematical modelers can develop models that can better analyze and predict the spread of infectious diseases. People can come into close contact in their workplaces. This report describes methods to develop georeferenced commuting patterns that can be used to characterize the work-related movement of US populations and help agent-based modelers predict workplace contacts that result in disease transmission. We used a census data product called "Census Spatial Tabulation: Census Track of Work by Census Tract of Residence (STP64)" as the data source to develop commuting pattern data for agent-based synthesized populations databases and to develop map products to visualize commuting patterns in the United States. The three primary maps we developed show inbound, outbound, and net change levels of inbound versus outbound commuters by census tract for the year 2000. Net change counts of commuters are visualized as elevations. The results can be used to quantify and assign commuting patterns of synthesized populations among different census tracts.

THE ESSENTIAL WORK IN TRAVEL MEDICINE -- NOW COMPLETELY UPDATED FOR 2018 As unprecedented numbers of travelers cross international borders each day, the need for up-to-date, practical information about the health challenges posed by travel has never been greater. For both international travelers and the health professionals who care for them, the CDC Yellow Book 2018: Health Information for International Travel is the definitive guide to staying safe and healthy anywhere in the world. The fully revised and updated 2018 edition codifies the U.S. government's most current health guidelines and information for international travelers, including pretravel vaccine recommendations, destination-specific health advice, and easy-to-reference maps, tables, and charts. The 2018 Yellow Book also addresses the needs of specific types of travelers, with dedicated sections on: · Precautions for pregnant travelers, immunocompromised travelers, and travelers with disabilities · Special considerations for newly arrived adoptees, immigrants, and refugees · Practical tips for last-minute or resource-limited travelers · Advice for air crews, humanitarian workers, missionaries, and others who provide care and support overseas Authored by a team of the world's most esteemed travel medicine experts, the Yellow Book is an essential resource for travelers -- and the clinicians overseeing their care -- at home and abroad.