This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology – New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.

This book focuses on the emerging non-invasive imaging technique of Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO). FLIO reveals unique information on retinal diseases, ranging from age-related macular degeneration and vascular diseases to hereditary retinal dystrophies. Fluorescence lifetimes enable the evaluation of disease progression before irreversible structural changes occur. The image acquisition is suitable for diagnostic purposes and follow-up examinations to investigate the natural course of disease, and to monitor the effects of possible therapies. This book fills the gap between available literature and gives state-of-the-art guidance on the principles of the FLIO technique, image acquisition, and data analysis. Written by a team of expert leaders within this field, this book will be relevant for scientists and clinicians with an interest in ophthalmoscopy.

High-speed anterior segment optical coherence tomography (OCT) offers a non-contact method for high resolution cross-sectional and three-dimensional imaging of the cornea and the anterior segment of the eye. As the first text completely devoted to this topic, Anterior Segment Optical Coherence Tomography comprehensively explains both the scientific principles and the clinical applications of this exciting and advancing technology. Anterior Segment Optical Coherence Tomography enhances surgical planning and postoperative care for a variety of anterior segment applications by expertly explaining how abnormalities in the anterior chamber angle, cornea, iris, and lens can be identified and evaluated using the Visante OCT(TM). Inside Anterior Segment Optical Coherence Tomography, Dr. Roger Steinert and Dr. David Huang, along with 22 of the field's leading professionals, provide a wealth of useful clinical and physiological material about this new diagnostic imaging technique. Valuable images are included to assist in the pre- and postoperative assessment of various anterior segment disorders. Additionally, this unique resource contains detailed information on biometric measurements to enhance diagnostic capability. On the leading edge of anterior segment imaging: - Mapping of corneal thickness and keratoconus evaluation - Measurement of LASIK flap and stromal bed thickness - Visualization and measurement of anterior chamber angle and diagnosis of narrow angle glaucoma - Measuring the dimensions of the anterior chamber and assessing the fit of intraocular lens implants - Visualizing and measuring the results of corneal implants and lamellar procedures - Imaging through corneal opacity to see internal eye structures With the increase in popularity of anterior chamber imaging, and anterior segment OCT proving to be the best tool for high resolution biometry, Anterior Segment Optical Coherence Tomography is a must-have for anterior segment, refractive, cornea, and glaucoma surgeons.

This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology - New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.

This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology - New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend. This work was published by Saint Philip Street Press pursuant to a Creative Commons license permitting commercial use. All rights not granted by the work's license are retained by the author or authors.

This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard. Contents Part I: Basics Brief history of fluorescence lifetime imaging The long journey to the laser and its use for nonlinear optics Advanced TCSPC-FLIM techniques Ultrafast lasers in biophotonics Part II: Modern nonlinear microscopy of live cells STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities Principles and applications of temporal-focusing wide-field two-photon microscopy FLIM-FRET microscopy TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing Laser tweezers are sources of two-photon effects Metabolic shifts in cell proliferation and differentiation Femtosecond laser nanoprocessing Cryomultiphoton imaging Part III: Nonlinear tissue imaging Multiphoton Tomography (MPT) Clinical multimodal CARS imaging In vivo multiphoton microscopy of human skin Two-photon microscopy and fluorescence lifetime imaging of the cornea Multiscale correlative imaging of the brain Revealing interaction of dyes and nanomaterials by multiphoton imaging Multiphoton FLIM in cosmetic clinical research Multiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgery Non-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse models Bedside assessment of multiphoton tomography

Optical coherence tomography (OCT) is the optical analog of ultrasound imaging and is emerging as a powerful imaging technique that enables non-invasive, in vivo, high resolution, cross-sectional imaging in biological tissue. This book introduces OCT technology and applications not only from an optical and technological viewpoint, but also from biomedical and clinical perspectives. The chapters are written by leading research groups, in a style comprehensible to a broad audience.

Successful thermal modeling of the human eye helps in the early diagnosis of eye abnormalities such as inflammation, cataracts, diabetic retinopathy, and glaucoma-all leading causes of blindness. This book presents a unified work of eye imaging and modeling techniques that have been proposed and applied to ophthalmologic problems. It delves into various morphological, texture, higher order spectra, and wavelet transformation techniques used to extract important diagnostic features from images, which can then be analyzed by a data scientist for automated diagnosis.

Optical Coherence Tomography gives a broad treatment of the subject which will include 1)the optics, science, and physics needed to understand the technology 2) a description of applications with a critical look at how the technology will successfully address actual clinical need, and 3) a discussion of delivery of OCT to the patient, FDA approval and comparisons with available competing technologies. The required mathematical rigor will be present where needed but be presented in such a way that it will not prevent non-scientists and non-engineers from gaining a basic understanding of OCT and the applications as well as the issues of bringing the technology to the market. - Optical Coherence Tomography is a new medical high-resolution imaging technology which offers distinct advantages over current medical imaging technologies and is attracting a large number of researchers. - Provides non-scientists and non-engineers basic understanding of Optical Coherence Tomography applications and issues.

Full-field optical coherence microscopy (FF-OCM) is an imaging technique that provides cross-sectional views of the subsurface microstructure of semitransparent objects. The technology is based on low-coherence interference microscopy, which uses an area camera for en face imaging of the full-field illuminated object. FF-OCM benefits from the lateral imaging resolution of optical microscopy along with the capacity of optical axial sectioning at micrometer-scale resolution. The technique can be employed in diverse applications, in particular for non-invasive examination of biological tissues. This handbook is the first to be entirely devoted to FF-OCM. It is organized into four parts with a total of 21 chapters written by recognized experts and major contributors to the field. After a general introduction to FF-OCM, the fundamental characteristics of the technology are analyzed and discussed theoretically. The main technological developments of FF-OCM for improving the image acquisition speed and for endoscopic imaging are presented in part II. Extensions of FF-OCM for image contrast enhancement or functional imaging are reported in part III. The last part of the book provides an overview of possible applications of FF-OCM in medicine, biology, and materials science. A comprehensive compilation of self-contained chapters written by leading experts, this handbook is a definitive guide to the theoretical analyses, technological developments, and applications of FF-OCM. Using the rich information the book is replete with, a wide range of readers, from scientists and physicists to engineers as well as clinicians and biomedical researchers, can get a handle on the latest major advances in FF-OCM.