Still widely used as gene markers, isozymes detected by zymogram techniques have proven valuable in a range of other biological applications over the last few years. Along with these new applications, many new techniques have also emerged. Yet more than eight years since the Handbook of Detection of Enzymes on Electrophoretic Gels was first publish

Methods enabling the direct study of genetic variation in natural populations have improved considerably. The new edition explores these updated techniques in DNA analysis and provides a revised and refined laboratory guide to investigating variation in DNA molecules.

Discussing all aspects connected with the scientific analysis of Rhipicephalus microplus, this book covers tick classification and identification, as well as methods of extracting natural products effective against ticks. It also describes tick cell culture procedures, tick acaricide-resistance diagnostics, and the identification of tick parasites and microorganisms from the host and the ticks’ fluids, as well as the diagnosis of Babesia and Anaplasma in R. microplus.

With millions of different bacterial species living in soil, the microbial community is extremely complex, varying at very small scales. Microbe-driven functions are essential for most processes in soil. Thus, a better understanding of this microbial diversity will be invaluable for the management of the various soil functions. Nucleic Acids and Proteins in Soil combines traditional approaches in soil microbiology and biochemistry with the latest techniques in molecular microbial ecology. Included are methods to analyse the presence and importance of nucleic acids and proteins both inside and outside microbial cells, the horizontal gene transfer which drives bacterial diversity, as well as soil proteomes. Further chapters describe techniques such as PCR, fingerprinting, the challenging use of gene arrays for structural and functional analysis, stable isotope probing to identify in situ metabolic functions, and the use of marker and reporter genes in soil microbial ecology.

This fifth edition of the successful, long-selling classic has been completely revised and expanded, omitting some topics on obsolete DNA electrophoresis, but now with a completely new section on electrophoretic micro-methods and on-the-chip electrophoresis. The text is geared towards advanced students and professionals and contains extended background sections, protocols and a trouble-shooting section. It is now also backed by a supplementary website providing all the figures for teaching purposes, as well as a selection of animated figures tested in many workshops to explain the underlying principles of the different electrophoretic methods.

Now in its third edition, this bestselling work continues to offer state-of-the-art information on the development and employment of capillary electrophoresis. With special emphasis on microseparations and microfluidics, it features new chapters describing the use of microchip electrophoresis and associated microtechniques, with a focus on the extraordinary breadth of work undertaken to expand CE methodologies in recent years. Enhanced by contributions from leading international experts, the Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition remains a seminal reference for the chemistry, biology, and engineering fields.

This Second Edition of the highly praised Cell Biology: A Laboratory Handbook brings together new and revised chapters. Each chapter is concisely written and beautifully illustrated, making the attractive four-volume set a worthwhile addition to any desktop, and the up-to-date instructions for biological techniques make this reference the next best thing to having the expert at your side. Dr. Julio Celis and the Associate Editors have drawn on peer review from the scientific community to include 40 percent new material in this much-needed and updated laboratory manual. In one easy to use reference, current and classic protocols are presented in a clear and reader-friendly format that makes this manual a necessity to undergraduate and graduate students as well as technicians and instructors. Key Features * Contains more than 40% new material * Provides cell biologists and other life scientists with the most up-to-date instructions for basic and advanced cell biological techniques, including those at the interface between cell and molecular biology * Features uniform style and editing and includes contributions from world-renowned authorities in their respective fields * Contains information appropriate for a large, diverse, and constantly growing international audience of cell, developmental, and molecular biologists, plus others who need these methods in their laboratory research * Includes color plates throughout the set for easy reference * Designed as the essential lab guide and research reference for the field

Probably the most ubiquitous biochemical method used today for examining the genetics of individuals, populations, or phylogenetic relationships between taxa is electrophoresis. This book has been created to offer a viewpoint regarding current electrophoretic separation methodologies of macromolecules and their major applications to fisheries management. The chapters in this book have been selected and organized into three sections to create a carefully blended mixture of methodologies and applications designed to educate the novice, as well as stimulate interest in professional researchers currently using electrophoresis for their work. The first section includes chapters that discuss the principles that explain the genetic basis of multiple molecular forms of proteins, the theory and practice of DNA analyses, and the methodology of electrophoretic separation of these macromolecules; starch gel electrophoresis as the predominant electrophoretic tool for fisheries genetics; and protein isoelectric focusing and DNA analysis. The second section describes a variety of applications for electrophoretic techniques. The third section presents a discussion and results of experiments conducted by Dennis Powers and his associates regarding the physiological significance of multiple forms of enzymes using the fish Fundulus heteroclitus as a model system. The book features a catalog of nearly 100 enzyme staining recipes and covers new areas in electrophoretic work, such as DNA fingerprinting, genetic tags, mitochondrial DNA methodologies, and genomic manipulation of fish stocks. This book will provide a useful reference resource for fisheries biologists at federal, state, and local levels; fisheries researchers at universities; and students pursuing degrees involving research in fish genetics.