Electron magnetic resonance spectroscopy is undergoing something akin to a renaissance that is attributable to advances in microwave circuitry and signal processing software. EPR: Instrumental Methods is a textbook that brings the reader up to date on these advances and their role in providing better experimental techniques for biological magnetic resonance. Chapters in this book guide the reader from basic principles of spectrometer design through the advanced methods that are providing new vistas in disciplines such as oximetry, imaging, and structural biology. Key Features: Spectrometer design, particularly at low frequencies (below X-band), Design of spectrometer components unique to ENDOR and ESEEM, Optimization of EMR spectrometer sensitivity spanning many octaves, Algorithmic approach to spectral parameterization, Application of Fourier Methods to polymer conformation, oximetry, and imaging.

Electron magnetic resonance spectroscopy is undergoing something akin to a renaissance that is attributable to advances in microwave circuitry and signal processing software. EPR: Instrumental Methods is a textbook that brings the reader up to date on these advances and their role in providing better experimental techniques for biological magnetic resonance. Chapters in this book guide the reader from basic principles of spectrometer design through the advanced methods that are providing new vistas in disciplines such as oximetry, imaging, and structural biology. Key Features: Spectrometer design, particularly at low frequencies (below X-band), Design of spectrometer components unique to ENDOR and ESEEM, Optimization of EMR spectrometer sensitivity spanning many octaves, Algorithmic approach to spectral parameterization, Application of Fourier Methods to polymer conformation, oximetry, and imaging.

Computational and Instrumental Methods in EPR is devoted to both instrumentation and computation aspects of EPR, while addressing applications such as spin relaxation time measurements. However, this is the first comprehensive volume to offer practical, non-invasive spectroscopic methods of analyzing the rheology of biopolymers: comparative studies of polymer fluidity using traditional methods (e.g. viscosity) and nuclear magnetic resonance.

Although originally invented and employed by physicists, electron paramagnetic resonance (EPR) spectroscopy has proven to be a very efficient technique for studying a wide range of phenomena in many fields, such as chemistry, biochemistry, geology, archaeology, medicine, biotechnology, and environmental sciences. Acknowledging that not all studies require the same level of understanding of this technique, this book thus provides a practical treatise clearly oriented toward applications, which should be useful to students and researchers of various levels and disciplines. In this book, the principles of continuous wave EPR spectroscopy are progressively, but rigorously, introduced, with emphasis on interpretation of the collected spectra. Each chapter is followed by a section highlighting important points for applications, together with exercises solved at the end of the book. A glossary defines the main terms used in the book, and particular topics, whose knowledge is not required for understanding the main text, are developed in appendices for more inquisitive readers.

Since its inception 50 years ago, electron paramagnetic resonance (EPR, also called ESR or EMR) has become a major tool in diverse fields ranging from biology and chemistry to solid state physics and materials science. This important book includes personal descriptions of early experiments by pioneers who laid the foundations for the field, perspectives on the state of the art, and glimpses of future opportunities. It presents a broad view of the foundations of EPR and its applications, and will therefore appeal to scientists in many fields. Even the expert will find here history not previously recorded and provocative views of future directions.

This book describes the methods of analysis and determination of oxidants and oxidative stress in biological systems. Reviews and protocols on select methods of analysis of ROS, RNS, oxygen, redox status, and oxidative stress in biological systems are described in detail. It is an essential resource for both novices and experts in the field of oxidant and oxidative stress biology.

Biological magnetic resonance (NMR and EPR) is a rapidly expanding area of research with much activity in most universities and research institutions. International conferences are held biennially with an increasing number of participants. With the introduction of sophisticated and continuously im proving instrumentation, biological magnetic resonance is approaching the state of a common physical method in biochemical, biomedical, and bio logical research. The lack of monograpbs on the subject had been con spicuous for a long time. This gap started to close only recently. However, because of the rapid expansion and intensive research, many texts are dated by the time of their appearance. Therefore we have undertaken the editing of a series that is intended to provide the practicing chemist, biochemist, or biologist with the advances and progress in selected contemporary topics. In seeking to make the series as authoritative as possible, we have invited authors who have not only made significant contributions but who are also currently active in their fields. We hope that their expertise as well as their first hand experience as reflected in the chapters of this volume will be of benefit to the reader, inter alia, in planning his own experiments and in critically evaluating the current literature.

Electron paramagnetic resonance (EPR) applications remain highly significant in modern analytical science and this volume compiles critical coverage of developments in the recent literature. The topics covered in this volume describe contrasting types of EPR application, including rapid scan EPR, using the EPR toolkit to investigate the structural dynamics of membrane proteins and pulse dipolar EPR spectroscopy for investigating biomolecular binding events. An additional chapter reviewing the PARACAT collaboration from the EU has also been included. Providing a snapshot of the area by a handpicked group of researchers at the cutting-edge of the field, this book is a useful addition to any library supporting this research.

Comprehensive, Up-to-Date Coverage of Spectroscopy Theory and its Applications to Biological SystemsAlthough a multitude of books have been published about spectroscopy, most of them only occasionally refer to biological systems and the specific problems of biomolecular EPR (bioEPR). Biomolecular EPR Spectroscopy provides a practical introduction t

This book covers the basic theory and techniques, as well as various applications of pulsed electron-electron double resonance (PELDOR or DEER). This electron paramagnetic resonance technique is able to measure the distances and the distribution of distances between electron spins in the 1.5-15 nanometer scale; to determine the geometry of spin-labeled molecules; to estimate the number of interacting spins in spin clusters; and to characterize the spatial distribution of paramagnetic centers. As a result, PELDOR is now a popular method in EPR spectroscopy, particularly in the context of biologically important systems and soft matter and is also applied to problems in physical chemistry, biochemistry, polymers, soft matter and materials. Enabling readers to gain an understanding of the fundamentals of the PELDOR methods and an appreciation of the opportunities PELDOR provides, the book helps readers solve their own physical and biochemical problems.