In the clinical microbiology laboratory, blood is a critical diagnostic sample that, in the majority of cases is sterile (or is it?). However, when microbes gain access to and multiply in the bloodstream, it can result in life-threatening illness including sepsis. Mortality rates from bloodstream infection and sepsis range from 25% to 80%, killing millions of people annually. Blood cultures are a vital technology used in the microbiology laboratory to isolate and identify microbes and predict their response to antimicrobial therapy. The Dark Art of Blood Cultures, edited by Wm. Michael Dunne, Jr., and Carey-Ann D. Burnham, surveys the entire field of blood culture technology, providing valuable information about every phase of the process, from drawing samples to culture methods to processing positive cultures. The Dark Art of Blood Cultures is organized around several major topics. History of blood culture methods. Details the timeline of blood culture methods from manual through automated and describes the technological development of the leading automated blood culture systems (Bactec, BacT/Alert, and VersaTREK). Manual and automated blood culture methods. Critiques manual and automated methods for setting up blood cultures for adult and pediatric patients. Detection of pathogens directly from blood specimens. Describes currently available CE marked and FDA-cleared commercial tests using both phenotypic and genotypic markers, including their strengths and limitations. The workflow of culturing blood. Includes best practices from specimen collection to culture system verification, processing positive cultures for microbe identification and antibiotic susceptibility determination, along with the epidemiology of positive blood cultures and the value of postmortem blood cultures. Microorganisms in the blood. Examines the concept of a blood microbiome in healthy and diseased individuals. The Dark Art of Blood Cultures is a resource that clinicians, laboratorians, lab directors, and hospital administrators will find engaging and extremely useful. If you are looking for online access to the latest clinical microbiology content, please visit www.wiley.com/learn/clinmicronow.

In 1920s Switzerland, vampire lovers Charlotte and Karl are drawn into turmoil as Godric Reiniger, a local filmmaker and activist with sinister ambitions, begins his rise to power. Meanwhile, fiery dancer Emil achieves his dream to partner the legendary ballerina and vampire Violette Lenoir – until his forbidden desire for her becomes an obsession. Rejected, spiralling towards madness, he seeks solace with a mysterious beauty, Fadiya. But she too is a vampire, with a hidden agenda. When Karl and Charlotte undertake the perilous journey to rescue Emil, they unearth secrets that threaten the very existence of vampire-kind.

Learn to Work with the Magick of the Dark Moon Shadow magick occupies a critical but often misunderstood role in the rich history of witchcraft. This book explores topics such as the ethical use of animal parts and bones, blood magick, dark moon energy, and other rarely discussed aspects of witchcraft. With a focus on ethically sourcing materials and suggestions for plant-based substitutions, author Kate Freuler provides much-needed information and hands-on techniques to help you strengthen your witchcraft practice, connect to nature, protect yourself (and your kith and kin), and know yourself in a deep way. Within these pages, you will also discover methods for hexing, scrying, sex magick, and working with dark deities in addition to the magickal use of graveyard dirt and performing spells to assist the crossing of a dying loved one. The shadow work explored in Of Blood and Bones reminds us that not everything is love and light, and that facing the dark side supports the quest to achieve spiritual wholeness.

“High stakes, big heart, and lots of Black Girl Magic…unputdownable.” —Aiden Thomas, New York Times bestselling author of Cemetery Boys A rich, dark urban fantasy debut following a teen witch who is given a horrifying task: sacrificing her first love to save her family’s magic. The problem is, she’s never been in love—she’ll have to find the perfect guy before she can kill him. After years of waiting for her Calling—a trial every witch must pass to come into their powers—the one thing Voya Thomas didn’t expect was to fail. When Voya’s ancestor gives her an unprecedented second chance to complete her Calling, she agrees—and then is horrified when her task is to kill her first love. And this time, failure means every Thomas witch will be stripped of their magic. Voya is determined to save her family’s magic no matter the cost. The problem is, Voya has never been in love, so for her to succeed, she’ll first have to find the perfect guy—and fast. Fortunately, a genetic matchmaking program has just hit the market. Her plan is to join the program, fall in love, and complete her task before the deadline. What she doesn’t count on is being paired with the infuriating Luc—how can she fall in love with a guy who seemingly wants nothing to do with her? With mounting pressure from her family, Voya is caught between her morality and her duty to her bloodline. If she wants to save their heritage and Luc, she’ll have to find something her ancestor wants more than blood. And in witchcraft, blood is everything.

A tale of passion, betrayal... and blood... On a First World War battlefield vampire Karl von Wultendorf struggles to free himself from his domineering maker, Kristian. The Neville sisters flourish in decadent, hedonistic London society in 1923: champagne, parties and the latest illegal substances. All except Charlotte, the middle of the three sisters who hides in a corner wishing she were back in Cambridge helping her professor father with his scientific experiments. When Charlotte meets her father's new research assistant Karl, it is the beginning of a deadly obsession that divides her from her sisters, her father and even her dearest friend. What price are they willing to pay to stay together? "Not merely one of the finest fantasy novels of recent years, but one of the finest ever. Should not be missed." Brian Stableford "A cross between Anne Rice and some of the more edgy modern paranormal romances, only with Freda Warrington 's incredible voice... This author truly has a gift for story telling." Not Your Ordinary Book Banter

Set during World War I, this monumental philosophical novel about human despair inspired Albert Camus' own writing and prefigured the greater existential movement. Blood Dark tells the story of a brilliant philosopher trapped in a provincial town and of his spiraling descent into self-destruction. Cripure, as his students call him—the name a mocking contraction of Critique of Pure Reason—despises his colleagues, despairs of his charges, and is at odds with his family. The year is 1917, and the slaughter of the First World War goes on and on, with French soldiers not only dying in droves but also beginning to rise up in protest. Still haunted by the memory of the wife who left him long ago, Cripure turns his fury and scathing wit on everyone around him. Before he knows it, a trivial dispute with a complacently patriotic colleague has embroiled him in a duel.

I'm Ayla Night, a regular girl with some seriously bad luck. When I receive a summons to the elite Dark Arts Academy, I know it has to be a mistake. I'm a human. A nobody. And yet... I have no soul. How the f-- did that happen? Once class starts, I manage to piss off all the major Clans just by breathing. The Pyros, the Necromancers, the Shades, and the Alchemists all want me dead. Because I'm a lost pupil of the Cursed Clan and it seems my luck is just about to turn. This is a reverse harem paranormal romance with steam, a snarky grimoire, and a badass FMC that will blow your mind.

For the love of her vampire suitor, Karl, Charlotte has forsaken her human life. Now her only contact with people is when she hunts them down to feed. The one light in the shadows is the passion that burns between her and Karl. A love that it seems will last for eternity - until Karl's former lover, the beautiful Katerina, returns from her grave in the icy depths of the Weisskalt to reclaim her life...and Karl.

Being a vampire isn't as glamorous as it sounds . . . Jack Fleming, Anerican journalist, was bitten by the wrong girl--and Jack joined the living dead. Eccentric detective Charles Escott helps Jack solve any strange cases that happen along . . . and now Jack and Charles investigate the case of the talented young artist whose life is suddenly cut short.