This book is aimed at scientists, technologists, engineers, and undergraduate and graduate students involved in analytical and process biochemistry and biotechnology. It reviews the potentialities of light-emitting reaction associated with the sensor approach.The book introduces the concepts of sensors and biosensors and places bio- and chemi-luminescent sensors in the general context of biosensors. It then briefly describes luminescence phenomena and provides some basic knowledge necessary for understanding and exploiting light-emitting reactions. These luminescence reactions, important from an analytical standpoint, are described. Also the applications of bio- and chemi-luminescence which make use of immobilized reagents are explained. Finally, there is discussion of bio- and chemi-luminescent sensors, most of them including fiber optics.

This book is aimed at scientists, technologists, engineers, and undergraduate and graduate students involved in analytical and process biochemistry and biotechnology. It reviews the potentialities of light-emitting reaction associated with the sensor approach.The book introduces the concepts of sensors and biosensors and places bio- and chemi-luminescent sensors in the general context of biosensors. It then briefly describes luminescence phenomena and provides some basic knowledge necessary for understanding and exploiting light-emitting reactions. These luminescence reactions, important from an analytical standpoint, are described. Also the applications of bio- and chemi-luminescence which make use of immobilized reagents are explained. Finally, there is discussion of bio- and chemi-luminescent sensors, most of them including fiber optics.

This book covers optical chemical sensing by means of optical waveguides, from the fundamentals to the most recent applications. The book includes a historical review of the development of these sensors, from the earliest laboratory prototypes to the first commercial instrumentations. The book reprints a lecture by the Nobel Laureate Charles Townes on the birth of maser and laser, which lucidly illustrates the development of new science and new technology.

Key features include: Self-assessment questions and exercises Chapters start with essential principles, then go on to address more advanced topics More than 1300 references to direct the reader to key literature and further reading Highly illustrated with 450 figures, including chemical structures and reactions, functioning principles, constructive details and response characteristics Chemical sensors are self-contained analytical devices that provide real-time information on chemical composition. A chemical sensor integrates two distinct functions: recognition and transduction. Such devices are widely used for a variety of applications, including clinical analysis, environment monitoring and monitoring of industrial processes. This text provides an up-to-date survey of chemical sensor science and technology, with a good balance between classical aspects and contemporary trends. Topics covered include: Structure and properties of recognition materials and reagents, including synthetic, biological and biomimetic materials, microorganisms and whole-cells Physicochemical basis of various transduction methods (electrical, thermal, electrochemical, optical, mechanical and acoustic wave-based) Auxiliary materials used e.g. synthetic and natural polymers, inorganic materials, semiconductors, carbon and metallic materials properties and applications of advanced materials (particularly nanomaterials) in the production of chemical sensors and biosensors Advanced manufacturing methods Sensors obtained by combining particular transduction and recognition methods Mathematical modeling of chemical sensor processes Suitable as a textbook for graduate and final year undergraduate students, and also for researchers in chemistry, biology, physics, physiology, pharmacology and electronic engineering, this bookis valuable to anyone interested in the field of chemical sensors and biosensors.

The chemical sensor plays an essential role in the fields of environmental conservation and monitoring, disaster and disease prevention, and industrial analysis. A typical chemical sensor is a device that transforms chemical information in a selective and reversible way, ranging from the concentration of a specific sample component to total composition analysis, into an analytically useful signal. Much research work has been performed to achieve a chemical sensor with such excellent qualities as quick response, low cost, small size, superior sensitivity, good reversibility and selectivity, and excellent detection limit. This book introduces the latest advances on chemical sensors. It consists of 15 chapters composed by the researchers active in the field of chemical sensors, and is divided into 5 sections according to the classification following the principles of signal transducer. This collection of up-to-date information and the latest research progress on chemical sensor will provide valuable references and learning materials for all those working in the field of chemical sensors.

Recognition receptors play a key role in the successful implementation of chemical and biosensors. Molecular recognition refers to non-covalent speci?c binding between molecules, one of which is typically a macromolecule or a molecular assembly, and the other is the target molecule (ligand or analyte). Biomolecular recognition is typically driven by many weak interactions such as hydrogen bo- ing, metal coordination, hydrophobic forces, van der Waals forces, pi-pi interactions and electrostatic interaction (due to permanent charges, dipoles, and quadrupoles) the polarization of charge distributions by the interaction partner leading to ind- tion and dispersion forces, and Pauli-exclusion-principle-derived inter-atomic repulsion, and a strong, “attractive” force arising largely from the entropy of the solvent and termed the hydrophobic effect. In recent years, there has been much progress in understanding the forces that drive the formation of such complexes, and how these forces are relate to the physical properties of the interacting molecules and their environment allows rational design of molecules and materials that interact in speci?c and desired ways. This book presents a signi?cant and up-to-date review of the various recognition elements, their immobilization, characterization techniques by a panel of dist- guished scientists. This work is a comprehensive approach to the recognition receptors area presenting a thorough knowledge of the subject and an effective integration of these receptors on sensor surfaces in order to appropriately convey the state-of the-art fundamentals and applications of the most innovative approaches.

This book offers a complete and well-organized review of the latest advances made in developing ultra-weak chemiluminescence techniques for analytical applications. It systematically introduces the current theories, mechanisms, instruments, technologies, and real applications of ultra-weak chemiluminescence. Compared to books devoted to the normal chemiluminescence and bioluminescence, this book covers a wide range of ultra-weak chemiluminescence based on inorganic chemical reactions and nanotechnology from a principle and practical point of view. This book is intended for readers who are interested in expanding their knowledge of chemiluminescence and employing ultra-weak chemiluminescence techniques to develop new detection methods for analytical applications.

Significant progress has been made in recent years in quenched-phosphorescence oxygen sensing, particularly in the materials and applications of this detection technology that are open to commercialization, like uses in brain imaging and food packaging. Prompted by this, the editors have delivered a dedicated book that brings together these developments, provides a comprehensive overview of the different detection methodologies, and representative examples and applications. This book is intended to attract new researchers from various disciplines such as chemistry, physics, biology and medicine, stimulate further progress in the field and assist in developing new applications. Providing a concise summary at the cutting edge, this practical guide for current experts and new potential users will increase awareness of this versatile sensing technology.

Flow-through sensors are more suitable than classical probe-type sensors for addressing real (non-academic) problems. The external shape and operation of flow-through (bio)chemical sensors are of great practical significance as they facilitate sample transport and conditioning, as well as calibration and sensor preparation, maintenance and regeneration, all of which result in enhanced analytical features and a wider scope of application. This is a systematic presentation of flow-through chemical and biochemical sensors based on the permanent or transient immobilization of any of the ingredients of a (bio)chemical reaction (i.e. the analyte, reagent, catalyst or product) where detection is integrated with the analytical reaction, a separation process (dialysis, gas diffusion, sorption, etc.) or both.The introductory chapter provides an overview of (bio)chemical sensors and their impact on analytical chemistry. Essential concepts of flow-through (bio)chemical sensors including their definition, classification, the types of flow-cells where the sensing microzone can be accommodated, continuous-flow configurations to which they can be coupled, the measurement modes available and the types of transient signals obtained, among others, are the subject of Chapter 2. The remaining chapters classify the most relevant types of flow-through (bio)chemical sensors according to the processes taking place at the sensing (recognition) microzone, as well as their position in space and time.The book deals critically with most types of flow-through sensors, discussing their possibilities and shortcomings to provide a realistic view of the state-of-the-art in the field. The large numbers of figures, the wealth of literature references and the extensive subject index complement the text.

With contributions by numerous experts