Electrogenerated chemiluminescence (ECL) is a powerful and versatile analytical technique, which is widely applied for biosensing and successfully commercialized in the healthcare diagnostic market. After introducing the fundamental concepts, this book will highlight the recent analytical applications with a special focus on immunoassays, genotoxicity, imaging, DNA and enzymatic assays. The topic is clearly at the frontier between several scientific domains involving analytical chemistry, electrochemistry, photochemistry, materials science, nanoscience and biology. This book is ideal for graduate students, academics and researchers in industry looking for a comprehensive guide to the different aspects of electrogenerated chemiluminescence.

Chemiluminescence is a fascinating and mesmerizing phenomenon, which consists on the generation of light as the result of a chemical reaction. This process has been gaining attention in the last decades, being now a standard tool in analytical, bioimaging, biomedical and pharmaceutical applications. This book provides a detailed overview of the basic mechanisms and principles of the most relevant chemiluminescent systems, as well as describing the most recent advances and applications. Written by experts, this is a reference text suitable both for researchers focused on chemiluminescence and for those wanting to start working on this field. In Chapter One, the progress and evolution of the chemiluminescent studies in imidazopyrazinone-based compounds are reviewed, with emphasis on their potential as diagnosis tools. Chapter Two addresses the spectral changes associated with firefly bioluminescence by focusing on the tautomeric equilibria of the light emitter. Chapter Three reviews, for the first time, the liquid-phase chemiluminescence of fullerene and derivatives. Chapter Four is devoted to studies of chemiluminescence with the participation of lanthanide ions. Chapter Five summarizes the mechanism behind the chemiluminescence of acridinium esters, while discussing their current applications. Chapter Six explains the potential of phenothiazine derivatives as enhancers in analytic chemiluminescent assays. Chapter Seven collects the available information of both well-known and lesser-known examples of squid bioluminescence. Chapter Eight discusses the enhancement of chemiluminescent emission by using metal-nanoparticles near a luminescent molecule, which is known as metal-enhanced chemiluminescence. In Chapter Nine it is explained how model molecules can make the study of complex bioluminescence reaction mechanisms accessible, as well as allowing for chemiluminescence on macroscopic molecular crystals. Chapter Ten describes experimental and computational approaches used for enhancing the thermostability of firefly luciferase. Chapter Eleven demonstrates the usefulness of theoretical studies for better understanding chemiluminescent reactions. Chapter Twelve describes the fundamental theoretical aspects of state-of-the-art multiconfigurational methods and shows illustrative examples of their application to the study of chemiluminescence. Finally, Chapter Thirteen discusses chemiluminescent experiments that are simple, inexpensive and can be performed by students.

This comprehensive overview of chemiluminescence and bioluminescence covers historical developments, fundamental principles, recent advances, instrumentation, and applications.

Theoretical and Computational Photochemistry: Fundamentals, Methods, Applications and Synergy with Experimental Approaches provides a comprehensive overview of photoactive systems and photochemical processes. After an introduction to photochemistry, the book discusses the key computational chemistry methods applied to the study of light-induced processes over the past decade, and further outlines recent research topics to which these methods have been applied. By discussing the synergy between experimental and computational data, the book highlights how theoretical studies could facilitate understanding experimental findings. This helpful guide is for both theoretical chemists and experimental photochemistry researchers interested in utilizing computational photochemistry methods for their own work. - Reviews the fundamentals of photochemistry, helping those new to the field in understanding key concepts - Provides detailed guidance and comparison of computational and theoretical methods, highlighting the suitability of each method for different case studies - Outlines current applications to encourage discussion of the synergy between experimental and computational data, and inspiring further application of these methods to other photochemical processes

The Czech Republic is one of the motherlands of beer culture – beers of the pilsner brewing tradition and the aromatic Saaz hops are famous the world over. Brewing technicians and scientists from the Czech Republic have an excellent reputation and are constantly seeking an exchange and discussion of their research findings on the international scene. And the team of authors around Professor Basařová are all experienced technicians and scientists with a wealth of international experience. "The Comprehensive Guide to Brewing" is a unique groundwork for brewing technicians which deals with all subject areas, from the raw materials to packaging. It also conveys advanced knowledge of the fundamentals of brewing research. Compulsory reading for anyone who wishes to gain in-depth knowledge of brewing technology.

Electrochemistry plays a key role in a broad range of research and applied areas including the exploration of new inorganic and organic compounds, biochemical and biological systems, corrosion, energy applications involving fuel cells and solar cells, and nanoscale investigations. The Handbook of Electrochemistry serves as a source of electrochemical information, providing details of experimental considerations, representative calculations, and illustrations of the possibilities available in electrochemical experimentation. The book is divided into five parts: Fundamentals, Laboratory Practical, Techniques, Applications, and Data. The first section covers the fundamentals of electrochemistry which are essential for everyone working in the field, presenting an overview of electrochemical conventions, terminology, fundamental equations, and electrochemical cells, experiments, literature, textbooks, and specialized books. Part 2 focuses on the different laboratory aspects of electrochemistry which is followed by a review of the various electrochemical techniques ranging from classical experiments to scanning electrochemical microscopy, electrogenerated chemiluminesence and spectroelectrochemistry. Applications of electrochemistry include electrode kinetic determinations, unique aspects of metal deposition, and electrochemistry in small places and at novel interfaces and these are detailed in Part 4. The remaining three chapters provide useful electrochemical data and information involving electrode potentials, diffusion coefficients, and methods used in measuring liquid junction potentials. * serves as a source of electrochemical information * includes useful electrochemical data and information involving electrode potentials, diffusion coefficients, and methods used in measuring liquid junction potentials * reviews electrochemical techniques (incl. scanning electrochemical microscopy, electrogenerated chemiluminesence and spectroelectrochemistry)

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"The Complete Guide to Lab Technician Work: Overview and Interview Q&A" is an essential resource for individuals looking to start a career as a lab technician or for those already working in the field who want to expand their knowledge and skills. This Lab Technician guidebook covers everything from lab safety and equipment usage to advanced laboratory techniques and emerging technologies. In Chapter 1, readers will learn about the role of a lab technician, the education and training required, and the necessary skills and attributes for success. Chapter 2 focuses on the importance of lab safety, including understanding chemical hazards, wearing personal protective equipment, and emergency procedures. Chapter 3 provides an in-depth exploration of laboratory equipment, including common instruments and their functions, proper usage and maintenance, and calibration and troubleshooting techniques. In Chapter 4, readers will learn about laboratory techniques, such as sample preparation, laboratory measurements and analysis, quality control, and data recording and reporting. Chapter 5 covers the basics of chemistry, including atomic structure and the periodic table, chemical bonding and reactions, acids and bases, and solutions and concentration calculations. In Chapter 6, readers will learn about microbiology, including microbial morphology and identification, culturing and isolation techniques, sterilization methods, and antimicrobial susceptibility testing. Chapter 7 focuses on hematology, including blood cell morphology and function, hematological disorders and disease states, laboratory testing for blood disorders, and blood transfusion protocols. In Chapter 8, readers will learn about immunology, including the immune system, antibody structure and function, immunoassay techniques, and diagnostic tests for immune disorders. Chapter 9 covers clinical chemistry, including analytical methods for measuring chemical constituents in biological samples, liver and kidney function tests, and lipid and glucose metabolism testing. In Chapter 10, readers will learn about laboratory management, including workflow optimization, quality management systems, inventory management, and personnel management and development. Chapter 11 explores emerging technologies in laboratory science, including advances in automation and robotics, point-of-care testing, precision medicine and personalized diagnostics, and the impact of artificial intelligence and machine learning. In Chapter 12, readers will find an extensive list of lab technician interview questions and answers to help them prepare for job interviews. Finally, in chapter 13, readers will learn about career paths and professional development opportunities for lab technicians, including advancement opportunities, continuing education and certification programs, networking, and professional organizations. Overall, "The Complete Guide to Lab Technician Work: Overview and Interview Q&A" provides readers with an exhaustive understanding of the role of a lab technician, essential knowledge and skills, and career development opportunities. Whether you are just starting out or looking to advance your career, this book is a must-read for anyone in the field of laboratory science.

Despite being a relatively straightforward clinical diagnosis, recognition of hidradenitis suppurativa (HS) is highly variable, and clinical management is challenging and complex. Written by the world's leading experts in HS, A Comprehensive Guide to Hidradenitis Suppurativa brings together up-to-date scientific evidence on the diagnosis, patho-mechanisms, comorbidities, and multi-faceted medical and surgical interventions for this debilitating condition—in one convenient reference. - Covers every aspect of this complex skin disorder: etiology, pathophysiology, epidemiology, medical, alternative therapies, a range of surgical options, laser treatments, and comorbidities. - Discusses specific patient populations such as children, women of childbearing potential, and pregnant and breastfeeding women. Because HS has higher prevalence in people of skin of color, this patient population is well-documented in the text. - Offers insights into multi-disciplinary care, patient support and education, patients at risk for rapid disease progression, and clinical and translational research. - Features procedural videos covering laser therapies, de-roofing procedures, excisions and closure techniques, cryoinsufflation techniques, and special wound care material selection and techniques. - Includes recent FDA-approved drugs as well as those drugs and therapies that show future promise. - Identifies evidence gaps that provide a springboard to the future innovations in HS care to come. - Edited and authored by global experts who have co-authored 2019 U.S. and Canadian guidelines on hidradenitis suppurativa.

Written to help companies comply with GMP, GLP, and validation requirements imposed by the FDA and regulatory bodies worldwide, Quality Control Training Manual: Comprehensive Training Guide for API, Finished Pharmaceutical and Biotechnologies Laboratories presents cost-effective training courses that cover how to apply advances in the life sciences