The Second Edition of this bestseller brings together basic plant pathology methods published in diverse and often abstract publications. The Second Edition is updated and expanded with numerous new figures, new culture media, and additional methods for working with a greater number of organisms. Methods are easy to use and eliminate the need to seek out original articles. This reference allows for easy identification of methods appropriate for specific problems and facilities. Scientific names of pathogens and some of their hosts are updated in this edition. The book also acts as a research source providing more than 1,800 literature citations. The Second Edition includes chapters on the following: Sterilization of culture apparatus and culture media Culture of pathogens with detailed techniques for 61 fungi and selected bacteria Long-term storage of plant pathogens Detection and estimation of inoculum for 28 soilborne fungal pathogens and 5 bacterial genera-15 methods for airborne inoculum and 13 methods for seedborne pathogens Establishment of disease and testing for disease resistance Work with soil microorganisms Fungicide evaluation Biological control Bright-field microscopy

Molecular Methods in Plant Pathology covers methods in phytopathology at the molecular level, including PCR techniques, electron microscopy, tissue culturing, and the cloning of disease-resistant genes. Phytopathologists, botanists, horticulturists, and anyone working in agriculture will find this a useful reference on biophysical, biochemical, biomolecular, and biotechnological methods.

Plant diseases can have an enormous impact on our lives. In a world where total crop failure can quickly lead to human misery and starvation, accurate diagnostics play a key role in keeping plants free from pathogens. In Plant Pathology: Techniques and Protocols, expert researchers provide methods which are vital to the diagnosis of plant diseases across the globe, addressing all three categories of plant pathology techniques: traditional, serological, and nucleic acid. Chapters examine recent and developing issues with crop identity and authenticity, allowing workers to genotype samples from two major food groups. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Authoritative and reader-friendly, Plant Pathology: Techniques and Protocols is an incredible guide which will soon prove to be indispensable, both to novices and expert researchers alike.

This invaluable resource introduces the eleven types of organism that cause plant disease, ranging from higher plants to viroids and describes examples of cash and staple crop diseases that have caused human catastrophes. Early chapters cover serological and molecular techniques for the diagnosis of plant pathogens, epidemiology, methods for estimating disease severity and its effect on crop yields and techniques for limiting inoculum. Later chapters are concerned with colonisation of the plant and symptom development and the underlying biochemical and genetic factors that control these events. Finally, the control of plant disease using a variety of techniques including genetic modification is discussed. Modern diagnostic techniques Epidemiology and the measurement of disease severity The biochemistry and molecular biology of plant disease Control through cultural, biological, genetic and molecular techniques A wealth of examples and applications including full colour photographs

Including reproducible laboratory protocols, this guide to fungal pathogens in plants has been written by expert researchers in the field and includes methods now used to study them, including techniques for model systems such as Arabidopsis thaliana.

Using molecular methods for plant disease diagnosis provides diagnosticians with a number of advantages over more traditional methods. They can allow the identification of morphologically similar species, for example, or the detection of infection prior to symptom formation. Not only can molecular tools help by increasing the efficacy, accuracy and speed of diagnosis; their common technological basis provides further benefits, especially where resources are limited and traditional skills are hard to sustain. This book provides protocols for nucleic acid-based methods currently applied to plant pathogen detection and identification. It takes the practitioner through the full range of molecular diagnostic and detection methods and, as these generic techniques are appropriate for use on any target with minimal modification, also provides a useful resource for students of plant pathology and plant pathologists. Beginning with the background and future directions of the science, it then addresses DNA barcoding, microarrays, polymerase chain reactions (PCR), quality assurance and more, forming a complete reference on the subject.

This textbook provides a comprehensive introduction to all aspects of plant diseases, including pathogens, plant-pathogen interactions, their management, and future perspectives. Plant diseases limit potential crop production and are responsible for considerable losses in agriculture, horticulture and forestry. Our global food production systems are under increasing pressure from global trade, climate change and urbanization. If we could alleviate the losses due to plant diseases, we would be able to produce roughly 20% more food - enough to feed the predicted world population in 2050. Co-authored by a group of international teachers of plant pathology who have collaborated for many years, the book gives expert and seamless coverage. Plant Pathology and Plant Diseases: Addresses major advances in plant-pathogen interactions, classification of plant pathogens, and the methods of managing or controlling disease Is relevant for a global audience; it covers many examples of diseases with an impact worldwide but with an emphasis on disease of particular importance in a temperate context Features over 400 striking figures and colour photographs It is suitable for graduate students and advanced undergraduates studying plant pathology, biology, agriculture and horticulture.

Plant Pathology presents information and advances in plant pathology including disease induction and development and disease resistance and control. This book is organized into two major parts encompassing 14 chapters that focus on diseases, pathogenicity, and pathogen variability. The first part of the book deals with general considerations of disease, the disease cycle, parasitism and pathogenicity, and the variability in pathogens. This is followed by a presentation of the mechanisms by which pathogens cause disease and plants resist disease. Core chapters focus on the effects of pathogen-produced enzymes, toxins, growth regulators, and polysaccharides on the structural organization and on the basic physiological processes of photosynthesis, translocation, and respiration. The chapters also discuss the defense mechanisms of the plant. Moreover, this book explains the genetics of host-parasite interaction, effects of environment on disease development, and control. The second part of the book deals with the infectious diseases caused by fungi, bacteria, parasitic higher plants, viruses, and nematodes. This part also looks into the noninfectious diseases caused by environmental factors. The diseases caused by each type of pathogen are discussed comprehensively as a group and are subsequently discussed individually in detail. This book includes diagrams of cycles for each disease to create visual images for better understanding of the disease and message retention. This book is ideal for students with introductory course in plant pathology.

Plant diseases play an important role on our daily lives. Most of plant diseases are visible and are caused by biotic and/or abiotic factors. Symptoms are usually the results of a morphological change, alteration or damage to plant tissue and/or cells due to an interference of the plant’s metabolism. All basic structures of vascular plants are subject to attack by pathogens. The failure in accurate disease diagnosis and management may lead to huge losses in plant production and related commodities, which causes nutritional food scarcity. Typically, the appearance of a biotic symptom will indicate the relatively late stage of an infection and/or colonization of a pathogen. Expert detection, accurate diagnosis, and timely management play a significant role in keeping plants free from pathogens. In this book expert scholars share their research knowledge and key literature which are vital toward the diagnosis of plant diseases across the globe, addressing traditional plant pathology techniques, as well as advanced molecular diagnostic approach.

Provides a concise and straightforward account of the historical development of the diverse and interwoven themes of infectious diseases of plants.