Microcolumn High-Performance Liquid Chromatography

Handbook of Chromatography features tables and chromatograms, theoretical discussions, and practical applications on the topic. Tables and chromatograms are based on polymer analyses abstracted from literature references dating from 1981-1991. Compounds presented in the tables and chromatograms include residual monomers, plasticizers, additives, antioxidants, and products from the thermal degradation (pyrolysis) of a broad range of synthetic polymers. Theoretical discussions focus on new developments in the respective areas of gas, pyrolysis-gas, liquid, and size exclusion chromatographic separations. Capillary column technology, inverse gas chromatography (IGC), supercritical fluid extractions (SFE), and supercritical fluid chromatography (SFC) are also covered. A Practical Applications subsection provides a list of commercial suppliers of column packings and packed columns for gas and liquid chromatography. The book will be an excellent reference for chromatographers, organic chemists, and analytical chemists.

Edited by two of the pioneers of microcolumn chromatography and written by recognized experts in the field, this book summarizes advances in microcolumn liquid chromatography, capillary supercritical fluid chromatography and microelectrophoresis. Its unique combination of expert knowledge from leading laboratories in the USA, Japan and Switzerland, results in a particularly in-depth and comprehensive coverage of the various aspects of microcolumn separation methods.

This book is the first of a two-volume project which attempts to treat the sample handling and detection processes in a liquid chromatographic system in an integrated fashion. The need for more selective and sensitive chromatographic methods to help solve the numerous trace analysis problems in complex samples is undisputed. However, few workers realize the strong interdependence of the various steps - sample handling, separation and detection - which must be considered if one wants to arrive at an optimal solution. By introducing a strong element of selectivity and trace enrichment in the sample preparation step, fewer demands are placed on the quality of the chromatography and often a simple UV detector can be used. By using a selective detection mode, i.e. a reaction detector, the sample handling step can frequently be simplified and more easily automated. The impact of such a ``total system'' approach on handling series of highly complex samples such as environmental specimens or biological fluids can be easily imagined.Each chapter includes sufficient references to the literature to serve as a valuable starting point for more detailed investigation. Special emphasis is placed on the sample handling, and high priority has been given to the treatment of chemical principles applied to the topics presented, rather than to instrumentation. Written by experienced practitioners, this volume will be of interest to investigators in many areas of application, including environmental scientists and those active in the clinical, pharmaceutical and bioanalytical fields.

Protein Liquid Chromatography is a handbook-style guide to liquid chromatography as a tool for isolating and purifying proteins, consisting of 25 individual chapters divided into three parts: Part A covers commonly-used, classic modes of chromatography such as ion-exchange, size-exclusion, and reversed-phase; Part B deals with various target protein classes such as membrane proteins, recombinant proteins, and glycoproteins; and Part C looks at various miscellaneous related topics, including coupling reaction, buffer solution additives, and software. The text as a whole can be viewed as a systematic survey of available methods and how best to use them, but also attempts to provide an exhaustive coverage of each facet. How to solve a specific problem using a chosen method is the overall essence of the volume. The principle philosophy of this compilation is that practical application is everything; therefore, both classical and modern methods are presented in detail, with examples involving conventional, medium- and high-pressure techniques. Over-exposure to history, concept, and theory has deliberately been avoided. The reader will find a wealth of tips and tricks from users for users, including advice on the advantages and disadvantages of each method. Easy-to-read sections on "Getting started now" and "Where to go from here" attempt to provide hands-on, fool-proof detailed practical procedures with complete and even standard model runs for any scientist or technician at work in this area.

Proceedings of the International Study Group for Tryptophan Research: Sixth International Meeting, held in Baltimore, Maryland, May 9--12, 1989

Principles, Materials and Techniques

A practical guide for chemists in the pharmaceutical industry to making automated analyses of drugs that will meet the standards of regulatory agencies. Reviews the standard techniques of high-performance liquid chromatography, specialized detection methods, automation in pharmaceutical analysis, analyses of pharmaceuticals- helping readers meet rigorous regulatory agency standards for acceptable test results. Written by leading experts in the field, this text describes current liquid chromatographic techniques in pharamaceutical analysis...discusses highly sensitve detailed detection of drugs... considers automatation in pharamaceutical analysis...examines new molecular entities and opitcal isomers... and more.

This is the first detailed description of method development in chromatography - the overall process of which may be summarized as: method selection, phase selection, selectivity optimization, and system optimization. All four aspects receive attention in this book.Chapter 1 gives a short introduction, describes chromatographic theory and nomenclature, and outlines the method development process. Chapter 2 describes guidelines for method selection, and quantitative concepts for characterizing and classifying chromatographic phases. Selective separation methods, from both gas and liquid chromatography are given in Chapter 3; the main parameters of each method are identified and simple, quantitative relations are sought to describe their effects. Criteria by which to judge the quality of separation are discussed in Chapter 4 with clear recommendations for different situations. The specific problems involved in the optimization of chromatographic selectivity are explained in Chapter 5. Optimization procedures, illustrated by examples, are extensively described and compared on the basis of a number of criteria. Suggestions are made both for the application of different procedures and for further research. The optimization of programmed analysis receives special attention in Chapter 6, and the last chapter summarizes the optimization of the chromatographic system, including the optimization of the efficiency, sensitivity and instrumentation.Those involved in developing chromatographic methods or wishing to improve existing methods will value the detailed, structured way in which the subject is presented. Because optimization procedures and criteria are described as elements of a complete optimization package, the book will help the reader to understand, evaluate and select current and future commercial systems.