This book provides a comprehensive overview on biotechnological applications of unicellular and multicellular fungi in a variety of industrial branches. Targeted genetic and metabolic engineering of fungi allows production of native and transgenic enzymes and proteins in industrial scales. Those most prominently find application in biorefineries for the production of value-added chemicals and biofuels, in the pharmaceutical industry as well as in biomedicine. Each chapter is dedicated to applications and potential beneficial use of particular strains of yeasts and filamentous fungi and their produced biomolecules. The book targets researchers from both academia and industry and graduate students working in microbial biotechnology.

An ideal starting point for any research study of filamentous fungi. • Incorporates the latest findings from such disciplines as physiology, taxonomy, genomics, molecular biology and cell biology. • Begins with an historical perspective, cell morphology and taxonomy, and moves on to such topics as cell growth, development, metabolism, and pathogenesis. • Presents the full range of the fungal kingdom and covers important topics as saprophytes, pathogens and endophytes. • Serves as a recommended text for graduate and undergraduate students.

This volume brings together detailed practical guidance from experienced researchers using genetic, genomic, cellular and biochemical methods, to attempt to determine the functions of genes and how they contribute to the biology of fungi.

The submersed cultivation of organisms in sterile containments or fermenters has become the standard manufacturing procedure, and will remain the gold standard for some time to come. This book thus addresses submersed cell culture and fermentation and its importance for the manufacturing industry. It goes beyond expression systems and integrally investigates all those factors relevant for manufacturing using suspension cultures. In so doing, the contributions cover all industrial cultivation methods in a comprehensive and comparative manner, with most of the authors coming from the industry itself. Depending on the maturity of the technology, the chapters address in turn the expression system, basic process design, key factors affecting process economics, plant and bioreactor design, and regulatory aspects.

Physiological and biochemical activities. Primary vs secondary metabolism. Laboratory equipment. Safety considerations. Culture and inoculation methods. Pigments enhancing media. Growth with inhibitory compounds. Carbon and nitrogen source assimilation. Enzymatic activity on solid media. Enzymatic activity of fungal extracts and brotys. Miscellaneous tests. Molecular biology methods: protein electrophoresis, DNA molecular biology. Cromatographic methods: Thin layer chromatography of secondary metabolites, HPLC, UV/VIS spectroscopy, isolation and purification of secondary metabolites, assessing biological activity.

SINCE THE EARLY DAYS OF MICROBIOLOGY IT HAS BEEN KNOWN THAT, during their life cycles, microorganisms exhibit developmental changes in common with other organisms. In the last decade interest in this aspect of microbiology has developed greatly, and research findings have provided an understanding of the genetic, molecular and biochemical bases of development. An important stimulus in this research has been the realis ation that microbial development, in its various forms, provides interest ing model systems that have relevance to a much wider understanding of the developmental processes in higher eukaryotes. Many undergraduate and other courses in microbiology reflect these developments. Up to now, the only source material for these courses has been symposia publications, or books of a more specialised nature and at an advanced level. The aim in writing this book, which is based on a series of undergraduate lectures given at the University of Nottingham, was to bring together the relevant aspects of the biology of microorganisms, in particular the bacteria and fungi. The algae and protozoa have been excluded, partly because of the limits of space and partly because they are very different from the bacteria and fungi in most aspects of their biology.

This book is about the growth and differentiation processes underlying the growth and differentia of filamentous fungi. The impetus for this work tion of fungi and that it provides the reader with stems from our perception that the coverage of adequate source references for further information. this highly diverse and important group of organ It is estimated conservatively that there are more isms has been neglected in recent years, despite than 1. 5 million species of fungi - more than five many significant advances in our understanding of times the number of vascular plants and second the underlying mechanisms of growth. This situ only in diversity to the insects. The extreme ation contrasts with the treatment of Saccharomyces diversity of form in the fungi has always been a cerevisiae, for example, which because of its ideal source of inspiration for mycologists. This book is properties for genetic analyses, has established concerned mainly with those systems that have itself as the model eukaryote for the analysis of the been well characterized from the biochemical, cell cycle, and basic studies of biochemical and physiological or genetic points of view. Although genetic regulation. This book does not deal with it has not been possible to illustrate the breadth of the detailed growth phYSiology of S.

In 1968 when Cell Walls and Membranes was published it was still reasonable to attempt to write a book covering the whole subject. Accordingly this edition of the book had something to say about walls from micro-organisms and plants as well as about membranes from bacteria and animal cells. A decade later this is manifestly impossible. Knowledge about almost all the subjects has grown explosively, par ticularly about membranes and the biosynthesis of macromolecules. Moreover aspects of the subject that were still in a relatively primitive state ten years ago have grown into highly sophisticated subjects worthy of extended treatment. The result is that the present book has had to be confined to structures and functions relating to only one division of the biological kingdom, namely micro-organisms. Even then severe limitations have had to be made to keep the task within the time available to the authors and their expertise. A few of the titles of chapters such as those on the isolation of walls and membranes, the structure of the components of bacterial and micro-fungal walls and their biosynthesis remain from the earlier book. These chapters have been almost completely rewritten and a number of quite new chapters added on topics such as the action of the antibiotics that inhibit bacterial wall syn thesis, on the function of bacterial membranes, and the bacterial autolysins.

This new edition of The Fungi provides a comprehensive introduction to the importance of fungi in the natural world and in practical applications, from a microbiological perspective.