Digitalization was just the beginning, the biggest revolution for mankind starts now Flying cars, autonomous driving, 3D-printed houses and lab-grown meat - what sounds like a science fiction movie, is already a reality. We are on the verge of a new age. Building upon digitalization, technologies like artificial intelligence, 5G, robotics, quantum computing and synthetic biology will bring profound changes to the world we know today. The impact on our everyday lives and our economy will be immense. The Internet-Era alone created companies worth over 7 trillion dollars - but only the US and China reaped the benefits. The upcoming era of exponential progress offers many new opportunities. Europe needs a mindset shift, a unique 10xDNA to take advantage of these prospects. 10xDNA will inform and inspire those who wish to understand how our world will change in the next decade. Instead of fearing the unknown, we should embrace the opportunities to build a strong, fair and diverse Europe. Are you ready to unleash your 10xFuture?

Magnetic Tweezers for the Study of Protein Structure and Function, Volume 694 in the Methods in Enzymology serial highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Single membrane protein tethering for magnetic tweezer experiments, Membrane protein folding studies using a robust magnetic tweezer method, Magnetic tweezers in cell mechanics, and more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology serials - Updated release includes the latest information on Magnetic Tweezers for the Study of Protein Structure and Function

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project” approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein—students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs*Student-tested labs proven successful in a real classroom laboratories*Exercises simulate a cloning project that would be performed in a real research lab*"Project" approach to experiments gives students an overview of the entire process*Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

A collection of readily reproducible methods for the design, preparation, and use of RNAs for silencing gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Covers research methods in biomineralization science - Contains sections on such topics as genome editing, genome engineering, CRISPR, Cas9, TALEN and zinc finger nuclease

Many executives, organisations and companies believe that the economic demands of the digital age can be met with diligence, skill and, above all, experience. They build on expertise, established structures and processes that they have been able to rely on for decades. They perfect the known methods and procedures and strive for the highest degree of flawlessness. However, they overlook the fact that striving for perfection is not only counterproductive, but even harmful. In times of digitalisation and the accompanying globalisation, products, goods and services are not constantly getting better, they are constantly getting different. The processes and business models that drive the economy are continuously changing. Those who focus on perfecting familiar methods, both in production, but even more so in organisational design and leadership, are training a racehorse and do not realise that they are competing against a Formula 1 racing car. Economics, management and human resources are based on 19th century theoretical foundations. They are perfected steam engines - smooth running, enduring in operation and elegant to look at. And completely unsuitable for flying to the moon. In short: they are representatives of the steampunk economy. In his book, Dr. Andreas Rein debunks the steampunk economy and impressively explains how to recognise it. He explains why all companies and organisations are affected by disruptive changes - regardless of sector and industry. He shows that in a digital, fully networked world, the goal must not be the moon, but at least Mars. In his passionate plea for unleashing innovative potential, he shows how to overcome the steampunk economy and create sustainability. For neither human nor earthly resources are unlimited.